Fluorescent probe of quantum dots and zinc oxide in a highly selective polymer simultaneously determined florfenicol and sparfloxacin

A nanocomposite fluorescent probe was fabricated for the simultaneous determination of florfenicol and sparfloxacin based on fluorescence quenching. The probe was synthesized by integrating nitrogen-doped graphene quantum dots (N-GQDs), cadmium telluride quantum dots (CdTe QDs) and zinc oxide nanoparticles (ZnO) into a molecularly imprinted polymer (MIP). The determination was based on the quenching of fluorescence emissions from N-GQDs by florfenicol, detected at 410 nm, and the quenching of fluorescence emissions from CdTe QDs by sparfloxacin, detected at 550 nm. The fluorescent probe was highly sensitive and specific with good linear relationships for florfenicol and sparfloxacin in the range  0.10 to 100.0 μg L^-1. The limits of detection for florfenicol and sparfloxacin were 0.06 and 0.10 μg L^-1, respectively. The fluorescent probe was used to determine florfenicol and sparfloxacin in food samples and the results agreed well with the results of chromatographic determination. Recoveries of spiked milk, egg and chicken samples reached 93.3-103.4% with good precision (RSD < 6%). The advantages of the nano-optosensor include high sensitivity and selectivity, simplicity, rapidity, convenience, good accuracy and precision.