Ecophysiological response of marine copepods to dietary elemental imbalances

We assessed the effects of nutrient imbalanced diets on the feeding, reproduction and gross-growth efficiency of egg production of the copepod Paracartia grani. The cryptophyte Rhodomonas salina, cultivated under balanced (f/2) and imbalanced growth conditions (N and P limitation), served as prey. Copepod C:N and C:P ratios increased in the imbalanced treatments, particularly under P limitation. Feeding and egg production rates did not differ between the balanced and N-limited treatments but decreased under P limitation. We found no evidence of compensatory feeding in P. grani. C gross-growth efficiency averaged 0.34 in the balanced treatment and declined to values of 0.23 and 0.14 for the N- and P-limited treatments, respectively. Under N limitation, N gross-growth efficiency increased significantly to a mean value of 0.69, likely as a result of increasing the nutrient absorption efficiency. P gross-growth efficiency reached values > 1 under P limitation, involving the depletion of body P. Hatching success was >80%, with no differences among diets. Hatched nauplii, however, had lower size and slower development when the progenitor was fed a P-limited diet. This study highlights the effects of P limitation in copepods, which are more constraining than N, and the presence of maternal effects driven by prey nutritional composition that ultimately may affect population fitness.