Biocompatible 7-nitro-2,1,3-benzoxadiazole-embedded naphthalimide for exploring endogenous H₂S in living cells

Hydrogen sulfide (H₂S) is a central signaling and antioxidant biomolecule involved in various biological processes. As inappropriate levels of H₂S in the human body are closely related to various diseases, including cancer, a tool capable of detecting H₂S with high selectivity and sensitivity in living systems is urgently required. In this work, we intended to develop a biocompatible and activatable fluorescent molecular probe for detecting H₂S generation in living cells. The 7-nitro-2,1,3-benzoxadiazole-imbedded naphthalimide (1) probe presented here responds specifically to H₂S and produces readily detectable fluorescence at 530 nm. Interestingly, probe 1 exhibited significant fluorescence responses to changes in endogenous H₂S levels as well as high biocompatibility and permeability in living HeLa cells. This allowed for the real-time monitoring of endogenous H₂S generation as an antioxidant defense response in the oxidatively stressed cells.