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Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield
L Krausser1, S M Braet2, Z Benaamar3
1Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; Research Foundation Flanders (FWO), Brussels, Belgium; University of Antwerp, Faculty of Medicine and Health Sciences, Antwerp, Belgium.
Diagnostic Microbiology and Infectious Disease
|March 11, 2023
Abstract:
Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.
