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Enzymatic determination of red cell creatine as an index of hemolysis
H Cramer1, H Dauwalder, H Meier
1Department of Clinical Chemistry, Inselspital, University of Berne, Switzerland.
Clinical Biochemistry
|October 1, 1987
Abstract:
An enzymatic assay for creatine, depending on the creatine kinase reaction, has been modified for the determination of creatine in packed erythrocytes, using a centrifugal analyzer (COBAS BIO). The method is precise, sensitive and shows excellent accuracy in recovery experiments when compared to the diacetyl-alpha-naphthol method. The enzymatic red cell creatine correlates with the erythrocyte survival time determined with the radioactive chromium method. It can be used as a rapidly available parameter for the quantification of hemolytic processes.