Efficient production of bacterial cellulose based composites using zein protein extracted from corn gluten meal

Corn gluten meal (CGM) which is a byproduct of corn wet milling is mainly used in animal and poultry feed. Due to its high protein content in CGM, it has been utilized for the extraction of zein protein which is the main hydrophobic protein present in the corn. The extracted zein protein was used along with bacterial cellulose that is highly pure, biocompatible, biodegradable, and generally regarded as safe for the preparation of composites that have better surface properties and applications. SEM analysis of the synthesized composite showed layering, incorporation of zein protein onto the surface of bacterial cellulose. XRD results showed there were no significant changes in the peak intensity due to the surface modification of BC membranes composites in comparison to pristine BC and TGA showed the thermostable characteristic of bacterial cellulose and are more capable of withstanding high temperature. Maximum production of bacterial cellulose was observed when corn gluten meal and zein protein were used as a cheap nitrogen sources for the production of bacterial cellulose along with other medium components. An increase of approximately 4.0 g/l of bacterial cellulose from 13.561 g/l to 17.83 g/l was observed when corn gluten meal and zein protein were used in the production medium. The prepared BC-based zein protein composites can be utilized for food packaging and storage applications.