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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
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TCR sequencing: applications in immuno-oncology research
Á F Sanromán1, K Joshi2,3, L Au1,4,5,6
1Cancer Dynamics Laboratory, The Francis Crick Institute, London, UK.
Immuno-Oncology Technology
|March 13, 2023
Summary
T-cell receptor (TCR) interactions drive anti-tumor responses. TCR sequencing reveals tumor-specific T cells, explaining how immunotherapies work through T-cell revival and replacement.
Area of Science:
- Immunology
- Oncology
- Genomics
Background:
- T-cell receptor (TCR) interaction with major histocompatibility complex-antigen complexes is crucial for initiating anti-tumor immune responses.
- Understanding the specific T cells that recognize tumor neoantigens is key to developing effective cancer immunotherapies.
Purpose of the Study:
- To explore the role of TCR sequencing in characterizing T cells that recognize tumor neoantigens.
- To investigate the mechanisms of T-cell clonal revival and replacement in the context of immunotherapy responses.
Main Methods:
- TCR sequencing analysis was employed to identify and characterize T-cell populations.
- The study focused on analyzing T-cell responses in relation to tumor antigens and immunotherapy.
Main Results:
- TCR sequencing enables the detailed characterization of T cells targeting specific tumor neoantigens.
- Evidence suggests that T-cell clonal revival and replacement are potential mechanisms driving the efficacy of cancer immunotherapies.
Conclusions:
- TCR sequencing is a powerful tool for dissecting anti-tumor T-cell responses.
- The dynamics of T-cell populations, including revival and replacement, are critical factors in successful cancer immunotherapy.
