Related Experiment Video
Updated: Aug 7, 2025
08:55
Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
Published on: December 13, 2021
2.3K
Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen
Chun Hao Wong1,2,3, Steven W Wingett1, Chen Qian4
1Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK.
Biorxiv : the Preprint Server for Biology
|March 13, 2023
Summary
Researchers identified novel regulators of the cytoplasmic dynein-1 motor, crucial for cellular organization and transport. This study reveals how SUGP1 sustains dynein activator LIS1, impacting microtubule-based transport.
Area of Science:
- Cell Biology
- Molecular Motors
- Genetics
Background:
- Cytoplasmic dynein-1 is essential for intracellular transport towards microtubule minus ends.
- The mechanisms governing dynein-1 biosynthesis, assembly, and functional regulation remain largely uncharacterized.
Approach:
- An arrayed CRISPR loss-of-function screen was performed in human cells.
- Dynein-tethered peroxisome and early endosome distribution served as phenotypic readouts.
- High-dimensional phenotypic data were clustered to identify co-functional genes.
Key Points:
- 195 validated genes impacting dynein function were identified from 18,253 screened genes.
- Genes were categorized based on their effects on multiple or subset of dynein cargoes.
- Novel candidate regulators of dynein function were discovered, including the RNA-binding protein SUGP1.
Conclusions:
- SUGP1 promotes dynein-mediated cargo trafficking by maintaining functional expression of the dynein activator LIS1.
- The findings provide a rich dataset for generating new hypotheses on microtubule-based transport and cellular organization.
- This study enhances understanding of the complex regulation of the dynein motor system.
