Related Experiment Video

Updated: Aug 7, 2025

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes
07:32

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

Published on: May 25, 2022

1.5K

Ectopic expression of SARS-CoV-2 S and ORF-9B proteins alters metabolic profiles and impairs contractile function in

Peng Zhang1, Yu Liu2, Chunfeng Li3

  • 1Department of Biological Sciences, Clemson University, Clemson, SC, United States.

Frontiers in Cell and Developmental Biology
|March 13, 2023
PubMed

Insights

The SARS-CoV-2 S and ORF-9B viral subunits impair heart cell function and metabolism. This study reveals mechanisms of COVID-19 cardiac complications, offering insights into viral impacts on the cardiovascular system.

Area of Science:

  • Cardiovascular Science
  • Molecular Biology
  • Virology

Background:

  • Coronavirus disease 2019 (COVID-19) presents cardiovascular complications.
  • The precise molecular mechanisms underlying these cardiac impacts remain largely unknown.

Purpose of the Study:

  • To investigate the toxic effects of SARS-CoV-2 subunits on human cardiomyocytes.
  • To elucidate the molecular mechanisms of COVID-19-associated cardiac dysfunction and metabolic alterations.

Main Methods:

  • Utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for pseudoviral infection with SARS-CoV-2 subunits.
  • Assessed cellular function, metabolic profiles, mitochondrial activity, and gene expression.
  • Performed transcriptomic analysis to identify dysregulated pathways.

Main Results:

  • Ectopic expression of S and ORF-9B subunits impaired cardiomyocyte contractile function and altered metabolic profiles.
  • Mitochondrial oxidative phosphorylation, membrane potential, and ATP production decreased; reactive oxygen species (ROS) increased with S subunit expression.
  • ORF-9B subunit overexpression led to elevated glycolysis and dysregulated metabolism-related signaling pathways, including HIF-signaling.

Conclusions:

  • SARS-CoV-2 S and ORF-9B subunits induce significant metabolic alterations and cardiac dysfunction in human cardiomyocytes.
  • These findings provide critical insights into the molecular basis of cardiovascular complications in COVID-19 patients.
Keywords:
9bSSARS-CoV-2cardiomyocytemetabolism

More Related Videos

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction08:31

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

Published on: July 26, 2018

10.2K
Isolation and Physiological Analysis of Mouse Cardiomyocytes11:02

Isolation and Physiological Analysis of Mouse Cardiomyocytes

Published on: September 7, 2014

23.2K

Related Concept Videos

Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
14
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
20
Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
14
Myocarditis I: Introduction01:21

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
14