Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.7K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.7K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
3.9K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

1.9K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.9K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

1.6K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The M1 Paradox: Pro-Tumorigenic Effect of Macrophage Cytotoxicity in Prostate Cancer.

International journal of molecular sciences·2026
Same author

Tumor-associated neutrophils in renal cell carcinoma.

Frontiers in immunology·2026
Same author

M1 macrophages - unexpected contribution to tumor progression.

Frontiers in immunology·2025
Same author

Detection of fusion events by RNA sequencing in FFPE versus freshly frozen colorectal cancer tissue samples.

Frontiers in molecular biosciences·2025
Same author

A New Approach of Detecting <i>ALK</i> Fusion Oncogenes by RNA Sequencing Exon Coverage Analysis.

Cancers·2024
Same author

Gene Expression and Pathway Activation Biomarkers of Breast Cancer Sensitivity to Taxanes.

Biochemistry. Biokhimiia·2024

Related Experiment Video

Updated: Aug 7, 2025

In Vitro Assay to Study Tumor-macrophage Interaction
08:36

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

7.6K

Macrophage - tumor cell interaction beyond cytokines.

Olga Kovaleva1, Maxim Sorokin1, Anastasija Egorova1

  • 1Laboratory for Tumor Stromal Cell Biology, Institute of Carcinogenesis, Nikolaj Nikolajevich (N.N.) Blokhin National Medical Research Center of Oncology, Moscow, Russia.

Frontiers in Oncology
|March 13, 2023
PubMed
Summary

Tumor cells and macrophages communicate via exosome-transported non-coding RNAs (ncRNAs), influencing tumor progression. This interaction offers promising avenues for novel cancer diagnostics and therapeutics, though further research is needed.

Keywords:
cancerexosomelncRNAmacrophagemiRNA

More Related Videos

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

7.6K
A Macrophage-Tumor Spheroid Co-Invasion Assay
09:01

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

700

Related Experiment Videos

Last Updated: Aug 7, 2025

In Vitro Assay to Study Tumor-macrophage Interaction
08:36

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

7.6K
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

7.6K
A Macrophage-Tumor Spheroid Co-Invasion Assay
09:01

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

700

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Tumor cell-macrophage communication is crucial for malignant potential.
  • Previous research focused on soluble factors, but exosomes and non-coding RNAs (ncRNAs) are now recognized as key mediators.
  • ncRNAs within exosomes play significant roles in regulating macrophage phenotype and activation.

Purpose of the Study:

  • To review the role of regulatory RNAs in macrophage phenotype development.
  • To examine the reciprocal influence between tumor cells and macrophages mediated by exosomal ncRNAs.
  • To highlight the potential of ncRNA-based exosome communication for cancer diagnostics and therapeutics.

Main Methods:

  • Literature review of studies on tumor-macrophage interactions.
  • Analysis of research on exosome-mediated transfer of ncRNAs (miRNA, lncRNA) between tumor and stromal cells.
  • Synthesis of findings on the impact of ncRNAs on macrophage polarization and tumor cell behavior.

Main Results:

  • ncRNAs are key regulators of macrophage phenotype and activation loops.
  • Exosomes carrying ncRNAs facilitate communication between tumor cells and tumor-associated macrophages (TAMs).
  • This intercellular ncRNA transfer significantly impacts tumor pathogenesis and stromal interactions.

Conclusions:

  • Exosomal ncRNAs represent a critical mechanism in tumor-stromal cell interaction.
  • The tumor cell-macrophage axis mediated by ncRNAs holds promise for developing novel cancer therapies and diagnostics.
  • Significant research gaps remain before clinical applications of ncRNAs can be realized.