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Related Experiment Video

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Effect of Selenium Nanoparticle Size on IL-6 Detection Sensitivity in a Lateral Flow Device.

Zoe Bradley1,2, Patrick A Coleman3,4, Melissa A Courtney3

  • 1Biopanda Reagents Ltd., Unit 14, Carrowreagh Business Park, Carrowreagh Road, Belfast BT16 1QQ, United Kingdom.

ACS Omega
|March 13, 2023
PubMed
Summary

New selenium nanoparticles (SeNPs) improve sepsis detection sensitivity in lateral flow devices (LFDs). Larger SeNPs offer a promising, more sensitive alternative for point-of-care sepsis diagnostics compared to gold nanoparticles.

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Area of Science:

  • Nanotechnology
  • Biomedical Diagnostics
  • Immunochemistry

Background:

  • Sepsis diagnosis is critical but challenged by slow, centralized testing.
  • Current point-of-care (POC) sepsis detection methods, like lateral flow devices (LFDs), lack sufficient sensitivity.
  • Interleukin-6 (IL-6) is a key biomarker for sepsis detection.

Purpose of the Study:

  • To develop and evaluate larger selenium nanoparticles (SeNPs) for enhanced sensitivity in LFD-based sepsis detection.
  • To compare the performance of SeNPs with commercial gold nanoparticles (AuNPs) in detecting the sepsis biomarker IL-6.
  • To investigate the impact of SeNP size on LFD assay sensitivity.

Main Methods:

  • Fabrication of LFDs utilizing 150-310 nm sized selenium nanoparticles (SeNPs).
  • Comparison of SeNP-based LFDs against commercial 40 nm gold nanoparticle (AuNP) LFDs.
  • Quantification of the limit of detection (LOD) for IL-6 using both SeNP and AuNP LFDs.

Main Results:

  • SeNP-based LFDs demonstrated a lower LOD for IL-6 (0.1 ng/mL) compared to AuNP-based LFDs (1 ng/mL).
  • Larger SeNPs (150-310 nm) achieved higher sensitivity than standard 40 nm AuNPs.
  • A trade-off was observed between sensitivity and test line visual intensity with SeNPs.

Conclusions:

  • Larger selenium nanoparticles are viable and potentially superior labels for LFDs in sepsis diagnostics.
  • SeNPs offer a promising avenue for improving the sensitivity of POC sepsis detection assays.
  • This study represents the first use of larger SeNPs (>100 nm) in LFDs for biomarker detection and compares their size-dependent effects on sensitivity.