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Mitochondrial morphology in Basidiobolus haptosporus.

A M Fiskin1, R G Garrison

  • 1Research Service, Veterans Administration Medical Center, Kansas City, Missouri 64128.

Cytobios
|January 1, 1987
PubMed
Summary

Mitochondria in the fungus Basidiobolus haptosporus form unusual goblet shapes, appearing as annulate views in thin sections. This morphology is linked to organelle associations, changing when grown on specific nitrogen sources.

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Area of Science:

  • Mycology
  • Cell Biology
  • Fungal Pathogenesis

Background:

  • Young hyphal cells of Basidiobolus haptosporus display unique mitochondrial structures.
  • These structures, termed annulate views, feature a central space with cytoplasmic components and other organelles.
  • The consistent morphology suggests specific organelle interactions rather than random mitochondrial plasticity.

Purpose of the Study:

  • To investigate the three-dimensional structure of unusual mitochondrial forms in Basidiobolus haptosporus.
  • To explore the relationship between mitochondrial morphology and cellular environment, particularly nitrogen sources.
  • To understand the spatial associations of organelles within young hyphal cells.

Main Methods:

  • Electron microscopy of thin sections of Basidiobolus haptosporus hyphae.
  • Three-dimensional reconstruction and projection of mitochondrial structures.
  • Culturing the fungus on xanthine or its catabolites as sole nitrogen sources for comparative analysis.

Main Results:

  • Three-dimensional projections suggest mitochondria adopt a goblet-shaped enclosure, with annulate views resulting from sectioning planes.
  • Organelle associations, including lipid bodies, other mitochondria, and microbodies, are frequently observed within these structures.
  • When grown on xanthine, microbody proliferation occurs, and annulate mitochondrial views disappear, with microbodies associating closely with mitochondria.

Conclusions:

  • The observed annulate mitochondrial views are a consequence of sectioning planes through goblet-shaped mitochondria, indicative of close organelle associations in young hyphae.
  • Cultivation on xanthine alters cellular composition, leading to microbody proliferation and a shift in mitochondrial morphology and associations.
  • These findings highlight the dynamic interactions between organelles in Basidiobolus haptosporus under varying nutritional conditions.

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