Related Experiment Video
Updated: Aug 7, 2025

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Published on: June 20, 2025
O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases
Aurore Labourel1, Jean-Luc Parrou1, Céline Deraison2
1TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
Inflammatory bowel diseases (IBD) involve gut bacteria degrading the mucus barrier using carbohydrate-active enzymes (CAZymes). Targeting these CAZymes with specific oligosaccharides may prevent IBD inflammation.
Area of Science:
- Microbiology
- Gastroenterology
- Biochemistry
Background:
- Inflammatory bowel diseases (IBD) are increasing globally, linked to gut microbiota changes and mucus barrier breaches by bacteria.
- Mucus, composed of glycans, is degraded by bacterial carbohydrate-active enzymes (CAZymes), crucial for bacterial invasion and IBD pathogenesis.
- Low-fiber diets are associated with IBD, while oligosaccharides like human milk oligosaccharides (HMOs) may competitively inhibit bacterial mucus degradation.
Conclusions:
- Understanding mucus-degrading CAZymes is vital for IBD research.
- Novel technologies can accelerate the discovery of these enzymes.
- Targeting CAZymes with specific oligosaccharides presents a promising therapeutic approach for IBD.
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