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Updated: Aug 7, 2025

Author Spotlight: A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay
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Author Spotlight: A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay

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A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay.

Saebom Kwon1, Jin I Lee2, Kyoung-Hye Yoon3

  • 1Department of Physiology, Mitohormesis Research Center, Yonsei University Wonju College of Medicine.

Journal of Visualized Experiments : Jove
|March 13, 2023
PubMed
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Researchers developed a cost-effective smartphone imaging method to track learned lawn avoidance behavior in Caenorhabditis elegans (C. elegans). This technique simplifies monitoring worm responses to harmful bacteria, saving time and resources.

Area of Science:

  • Behavioral Neuroscience
  • Biotechnology
  • Genetics

Background:

  • Caenorhabditis elegans (C. elegans) exhibits learned lawn avoidance behavior when exposed to toxic bacteria.
  • This behavior is crucial for studying responses to harmful environmental cues.
  • Traditional methods for quantifying this behavior are time-consuming and inconvenient.

Purpose of the Study:

  • To develop a cost-effective and accessible imaging method for assaying C. elegans learned lawn avoidance.
  • To enable researchers to efficiently monitor worm behavior over extended periods.
  • To provide an alternative to expensive imaging systems.

Main Methods:

  • Utilized a smartphone and an LED light box for transmitted light imaging.
  • Employed free time-lapse camera applications to capture images of multiple plates.

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  • Processed video files into manageable formats for manual counting of worms outside the bacterial lawn.

    • Main Results:

    • The smartphone-based method provides sufficient image quality for manual worm counting.
    • The system can image up to six plates simultaneously.
    • The processed video files are optimized for efficient data analysis.

    Conclusions:

    • This smartphone imaging technique offers a low-cost solution for studying C. elegans avoidance behavior.
    • The method is practical for researchers with limited resources.
    • The approach has potential applications for other C. elegans-based assays.