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Related Concept Videos

Protein Families02:47

Protein Families

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Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key...
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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Conservation of Protein Domains Over Different Proteins

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
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An Integrated Approach for Microprotein Identification and Sequence Analysis
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Searching and Navigating UniProt Databases.

Yvonne C Lussi1, Michele Magrane1, Maria J Martin1

  • 1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Cambridge, United Kingdom.

Current Protocols
|March 13, 2023
PubMed
Summary
This summary is machine-generated.

Learn to navigate and search the Universal Protein Resource (UniProt) database, a vital resource for protein sequence and annotation data. This guide details basic and advanced search strategies for UniProt datasets.

Keywords:
UniProtdatabasenavigationproteinsearchtutorial

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Area of Science:

  • Bioinformatics
  • Proteomics
  • Molecular Biology

Background:

  • The Universal Protein Resource (UniProt) is a critical global resource for protein sequence and functional annotation data.
  • The UniProt website serves as the primary access point, attracting approximately 800,000 unique visitors monthly.
  • It offers 10 searchable datasets and four main tools for protein data exploration.

Purpose of the Study:

  • To provide a comprehensive guide on utilizing UniProt datasets effectively.
  • To detail navigation and searching mechanisms within the UniProt website.
  • To explain advanced search and query-building techniques for accessing specific protein information.

Main Methods:

  • The article presents a series of protocols for using UniProt datasets.
  • Basic Protocol 1 covers fundamental navigation and searching techniques.
  • Protocols 2 and 3 build upon the basic protocol to describe advanced search functionalities and parameter utilization.

Main Results:

  • Users can efficiently search and retrieve protein data using UniProt's structured datasets.
  • Advanced search capabilities allow for precise querying and data filtering.
  • The protocols facilitate deeper exploration of protein information, including annotations, literature, taxonomy, and subcellular localization.

Conclusions:

  • This guide empowers researchers to effectively leverage UniProt datasets for their specific needs.
  • Mastering UniProt search functionalities enhances the accessibility and utility of protein information.
  • The described protocols support efficient data retrieval for various biological research applications.