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Related Experiment Videos

Plasma fluorescein decay determination during fluorophotometry.

J P Boot1, J A Van Best, E W Tjin a Tsoi

  • 1Laboratory of the Department of Ophthalmology, University Hospital, Leiden, the Netherlands.

Documenta Ophthalmologica. Advances in Ophthalmology
|April 1, 1987
PubMed
Summary

Two methods accurately approximate non-protein bound fluorescein (NPBF) decay curves in plasma using just two blood samples. Method 1 showed slightly better accuracy for NPBF concentration and plasma integral calculations.

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Area of Science:

  • Pharmacokinetics and Drug Metabolism
  • Biomedical Engineering
  • Ophthalmology

Background:

  • Accurate determination of drug decay curves in plasma is crucial for pharmacokinetic studies.
  • Non-protein bound fluorescein (NPBF) is a key indicator for assessing blood-retinal barrier permeability.
  • Existing methods for NPBF decay curve determination can be time-consuming and require frequent sampling.

Purpose of the Study:

  • To describe and evaluate two novel, simplified methods for determining the decay curve of NPBF in plasma.
  • To assess the accuracy of these methods using minimal blood samples.
  • To compare the accuracy of these methods with traditional approaches.

Main Methods:

  • Two mathematical models were developed: Method 1 uses a sum of two exponential decay functions, and Method 2 uses a power of time function.

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  • Parameters for both models were calculated using plasma NPBF concentrations from two samples taken at approximately 5 and 60 minutes post-injection.
  • Method accuracy was validated in 7 volunteers by comparing calculated concentrations and time integrals with values derived from 15-28 frequent blood samples.

    • Main Results:

    • Method 1 yielded a mean relative deviation of 9.2% +/- 4.3 SD for NPBF concentration, while Method 2 showed 12.7% +/- 4.5 SD.
    • For plasma integral calculations, Method 1 had a mean relative deviation of 2.8% at 15 min and 11% at 60 min.
    • Method 2 showed higher deviations: 17% at 15 min and 18% at 60 min.
    • Glucuronidation was estimated to affect blood-retinal barrier permeability calculations by a maximum of 20% for average glucuronidation percentages of 70% or less.

    Conclusions:

    • Both methods provide a useful approximation of NPBF decay curves with significantly fewer samples than traditional methods.
    • Method 1 demonstrates superior accuracy in predicting NPBF concentration and plasma integral values.
    • These simplified methods can enhance the efficiency of pharmacokinetic studies and blood-retinal barrier permeability assessments.