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Related Experiment Video

Updated: Aug 7, 2025

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Transcriptomic-based analysis to identify candidate genes for blue color rose breeding.

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  • 1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

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|March 13, 2023
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Summary

Researchers identified key genes, RhF3'H and RhGT74F2, responsible for the blue-purple flower color in

Keywords:
AnthocyaninMutationRhF3'HRhGT74F2RoseTranscriptome

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Area of Science:

  • Plant molecular biology
  • Genomics
  • Biochemistry

Background:

  • Roses (Rosa hybrida) exhibit diverse flower colors but lack natural blue varieties.
  • The genetic basis for blue-purple flower coloration in roses remains largely unelucidated.
  • Understanding rose color formation is crucial for breeding ornamental varieties.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying blue-purple flower color in the rose variety 'Rhapsody in Blue'.
  • To identify key genes involved in anthocyanin biosynthesis and accumulation in blue-purple rose petals.
  • To compare gene expression profiles between blue-purple and white petals to uncover color-determining factors.

Main Methods:

  • Transcriptome analysis (RNA-Seq) of blue-purple (BF) and white (WF) petals from 'Rhapsody in Blue'.
  • Differential gene expression analysis to identify significant gene expression changes.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
  • Quantitative real-time PCR (qRT-PCR) for gene expression validation.
  • Transient overexpression assays to functionally verify candidate genes.

Main Results:

  • Significantly higher anthocyanin content was observed in blue-purple petals compared to white petals.
  • 1077 differentially expressed genes (DEGs) were identified between BF and WF petals.
  • Genes RhF3'H and RhGT74F2 were found to be significantly up-regulated in BF petals and functionally verified to affect anthocyanin accumulation.

Conclusions:

  • RhF3'H and RhGT74F2 are key regulators in the formation of blue-purple flower color in 'Rhapsody in Blue'.
  • Transcriptome data provides comprehensive insights into rose color formation, particularly for blue hues.
  • This study lays the groundwork for understanding and potentially engineering blue roses.