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Development of Rat Caries-Induced Pulpitis Model for Vital Pulp Therapy.

H Huang1, M Okamoto1, M Watanabe1

  • 1Department of Restorative Dentistry and Endodontology, Graduate School of Dentistry, Osaka University, Suita-shi, Osaka, Japan.

Journal of Dental Research
|March 13, 2023
PubMed
Summary

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This summary is machine-generated.

Researchers developed a new rat model for dental caries-induced pulpitis to study vital pulp therapy. This model shows M2 macrophages are crucial for wound healing in reversible pulpitis after pulp capping.

Area of Science:

  • Dental research
  • Immunology
  • Regenerative medicine

Background:

  • Rodent models are vital for vital pulp therapy research due to anatomical and cellular similarities with humans.
  • Existing models often use uninfected teeth, limiting the assessment of inflammatory responses post-therapy.
  • A caries-induced pulpitis model is needed to better evaluate inflammatory shifts and healing after vital pulp therapy.

Purpose of the Study:

  • To establish a caries-induced pulpitis model in rats.
  • To evaluate inflammatory changes during wound healing after pulp capping in reversible pulpitis.
  • To investigate the role of macrophages in the healing process.

Main Methods:

  • Established a caries-induced pulpitis model using conventional rat caries model.
Keywords:
dentinogenesisdirect pulp cappingirreversible pulpitismacrophage polarizationreversible pulpitiswound healing

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  • Utilized immunostaining for inflammatory biomarkers like Toll-like receptor 2 and proliferating cell nuclear antigen.
  • Analyzed macrophage polarization (M1 vs. M2) at different caries stages and during wound healing.

    • Main Results:

    • Toll-like receptor 2 and proliferating cell nuclear antigen expression confirmed immune reactions in moderate and severe caries.
    • M2 macrophages predominated in moderate caries (reversible pulpitis), while M1 macrophages were prevalent in severe caries (irreversible pulpitis).
    • Pulp capping in reversible pulpitis resulted in complete tertiary dentin formation within 28 days; impaired healing was noted in irreversible pulpitis.

    Conclusions:

    • A successful caries-induced pulpitis model for vital pulp therapy research was established.
    • M2 macrophages play a significant role in the early wound-healing stages of reversible pulpitis.
    • The model allows for better investigation of inflammatory processes and therapeutic outcomes in infected dental pulp.