Related Experiment Video
Updated: Aug 7, 2025
08:02
A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
Published on: March 24, 2023
3.7K
A Mouse Model of MC903-Induced Atopic Dermatitis
Md Jahangir Alam1, Liang Xie1, Yu-Anne Yap2
1Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Current Protocols
|March 13, 2023
Summary
This study details a mouse model using MC903 to mimic atopic dermatitis (AD) inflammation. Researchers can use this model to assess skin thickness, itch, and immune cell infiltration for new AD therapies.
Area of Science:
- Immunology
- Dermatology
- Pharmacology
Background:
- Atopic dermatitis (AD) is a chronic inflammatory skin condition impacting many children, with unclear pathogenesis and no cure.
- Preclinical mouse models are vital for understanding AD and testing new treatments.
- The MC903-induced AD mouse model closely mimics human AD phenotypes with minimal systemic effects.
Purpose of the Study:
- To provide a detailed protocol for the MC903-induced AD mouse model.
- To outline methods for assessing AD-like skin inflammation and immune responses.
- To facilitate research into AD pathobiology and therapeutic development.
Main Methods:
- Topical application of MC903 to induce AD-like skin inflammation in mice.
- Measurement of skin thickness as an indicator of inflammation.
- Assessment of itch behaviors.
- Histological evaluation of skin tissue.
- Preparation of single-cell suspensions for flow cytometry analysis of immune cells.
Main Results:
- The MC903 model effectively induces AD-like skin inflammation.
- Functional measurements like skin thickness and itch can be reliably assessed.
- Histology reveals structural changes consistent with AD.
- Flow cytometry allows detailed analysis of immune cell infiltration.
Conclusions:
- The MC903-induced AD mouse model is a valuable tool for studying AD.
- This protocol enables comprehensive assessment of AD-like inflammation and immune responses.
- The model supports the evaluation of novel small molecule and antibody therapies for atopic dermatitis.
