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Phosphatidylseryl-transfer RNA in Bacillus licheniformis 749/C
Biochimica Et Biophysica Acta
|May 24, 1979
Summary
Phosphatidylserine is linked to transfer RNA (tRNA) in Bacillus licheniformis, particularly under alkaline conditions. This phospholipid can be synthesized and bound to tRNA in bacterial extracts, suggesting a functional role.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Phosphatidylserine (PS) presence in Bacillus licheniformis extracts is condition-dependent.
- PS was identified within the transfer RNA (tRNA) fraction.
Purpose of the Study:
- To investigate the association of phosphatidylserine with tRNA.
- To explore the synthesis and binding of PS to tRNA in bacterial systems.
Main Methods:
- Extraction and purification of tRNA from Bacillus licheniformis.
- pH manipulation to induce phospholipid release from tRNA.
- In vitro synthesis assays using CTP, phosphatidic acid, and S-30 extracts.
- DEAE-Sephadex chromatography for tRNA separation.
- Deaminoacylation and rechromatography to analyze tRNA species.
Main Results:
- Phosphatidylserine was exclusively released from tRNA at alkaline pH (9.0).
- Incubation with CTP, phosphatidic acid, and S-30 extracts increased bound PS on tRNA.
- Phosphatidylserine-bound tRNA was separated using DEAE-Sephadex chromatography.
- Analysis revealed that multiple tRNA isoaccepting species can carry phosphatidylserine.
Conclusions:
- Phosphatidylserine is covalently or tightly associated with tRNA in Bacillus licheniformis.
- Bacterial extracts possess the enzymatic machinery to synthesize and attach PS to tRNA.
- The binding of PS to tRNA is not limited to a single tRNA type, suggesting broader implications.