Three recombinant peroxidases as a degradation agent of aflatoxin M₁ applied in milk and beer

The aim of this work was to estimate the effects of three recombinant peroxidases (rPODs) on the degradation of aflatoxin M₁ (AFM₁) in a model solution and were applied in milk and beer to study the AFM₁ degradation. Besides, the contents of AFM₁ in model solution, milk and beer were evaluated, and the kinetic parameters of rPODs were determined (Michaelis-Menten constant - K_m and maximal velocity - V_max). The optimized reaction conditions (The degradation was over 60 %) for these three rPODs in the model solution were, respectively as follows: pH were 9, 9, and 10; hydrogen peroxide concentrations were 60, 50, and 60 mmol/L; at an ionic strength of 75 mmol/L and reaction temperature of 30 °C with 1 mmol/L K⁺ or 1 mmol/L Na⁺. These three rPODs (1 U /mL) presented the maximum activity for degradation of AFM₁ of 22.4 %, 25.6 %, and 24.3 % in milk, while 14.5 %, 16.9 %, and 18.2 % in beer, respectively. Meanwhile, the survival rate of Hep-G2 cells raised about 1.4 times after treated with peroxidase-generated AFM₁ degradation products. Therefore, POD may be a promising alternative to reduce the pollution of AFM₁ in model solution, milk, beer, and minimize their impact on the environment in humans.