Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbial Synergism Couples Root Metabolic Remodeling with Exudation Dynamics in <i>Liquidambar formosana</i>.

Microorganisms·2026
Same author

Transvaginal robotic-assisted natural orifice laparoscopic hysterectomy for benign gynecologic disease: Determining appropriate candidates.

Taiwanese journal of obstetrics & gynecology·2025
Same author

Redefining hysterectomy: Robotic versus conventional approaches in transvaginal natural orifice transluminal endoscopic surgery and laparoscopically-assisted vaginal hysterectomy.

Journal of the Formosan Medical Association = Taiwan yi zhi·2025
Same author

Factors affected the ovarian reserve after laparoscopic cystectomy for ovarian endometriomas.

European journal of obstetrics, gynecology, and reproductive biology·2024
Same author

Evaluation of a novel vaginal cells self-sampling device for human papillomavirus testing in cervical cancer screening: A clinical trial assessing reliability and acceptability.

Bioengineering & translational medicine·2024
Same author

Consistency in human papillomavirus type detection between self-collected vaginal specimens and physician-sampled cervical specimens.

Journal of medical virology·2024

Related Experiment Video

Updated: Aug 7, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.8K

Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing.

Yang Xu1, Cui-Yu Liu1, Wen-Qiang Cheng1

  • 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No. 76, Daqiao Road, Fuyang District, Hangzhou, China.

BMC Genomic Data
|March 14, 2023
PubMed
Summary

This study presents a comprehensive transcriptome database for Diospyros oleifera fruit development. This resource aids in understanding the complex regulation of sugar and tannin during persimmon fruit maturation.

Keywords:
Diospyros oleiferaFruit developmentFull-length transcriptomeNanopore sequencing

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.7K

Related Experiment Videos

Last Updated: Aug 7, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.8K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.7K

Area of Science:

  • Plant Biology
  • Genomics
  • Transcriptomics

Background:

  • Diospyros oleifera is crucial for persimmon fruit development research.
  • A lack of whole-transcriptome data has limited understanding of sugar and tannin regulation.

Purpose of the Study:

  • To generate a comprehensive full-length transcriptome database for Diospyros oleifera fruit at six developmental stages.
  • To provide a foundation for investigating transcript structure, variants, and evolution in persimmon.

Main Methods:

  • Utilized Oxford Nanopore Technologies for transcriptome sequencing of D. oleifera fruit.
  • Performed full-length transcriptome sequencing, generating 55.87 Gb of clean data.
  • Mapped data to the D. oleifera reference genome to identify transcripts, gene loci, and regulatory elements.

Main Results:

  • Obtained 51,588 full-length collapsing transcripts, including 2,727 new gene loci.
  • Functionally annotated 38,086 new transcripts.
  • Predicted 972 long non-coding RNAs (lncRNAs) and 7,159 alternative splicing (AS) events.

Conclusions:

  • A novel transcriptome database for D. oleifera fruit development is now available.
  • This resource facilitates deeper investigation into persimmon transcriptomics.
  • Enables future studies on the genetic mechanisms underlying fruit development and quality traits.