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Related Experiment Videos

Human 27-kDa calbindin complementary DNA sequence. Evolutionary and functional implications.

M Parmentier1, D E Lawson, G Vassart

  • 1Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Université Libre de Bruxelles, Belgium.

European Journal of Biochemistry
|December 30, 1987
PubMed
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Researchers identified human calbindin, a highly conserved protein with calcium-binding domains. Its evolutionary stability suggests a crucial role in protein interactions, potentially originating from an ancient ancestor.

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Biochemistry

Background:

  • Calbindin is a calcium-binding protein found in various species.
  • Understanding its structure and evolution can reveal its physiological functions.

Purpose of the Study:

  • To characterize human 27-kDa calbindin at the molecular and evolutionary level.
  • To investigate the evolutionary conservation and potential function of calbindin domains.

Main Methods:

  • Antibody screening of lambda gt11 brain libraries to isolate human calbindin cDNA clones.
  • DNA sequencing to determine the protein's amino acid sequence and domain structure.
  • Comparative analysis with homologous proteins from chick and bovine species.

Main Results:

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  • Human calbindin cDNA codes for a 261-amino acid protein with four active and two modified calcium-binding domains.
  • The protein exhibits high evolutionary conservation across species, with an evolutionary rate of 0.3 x 10(-9) amino acids/year.
  • Both active and inactive domains are equally conserved, suggesting functional importance beyond calcium binding.

Conclusions:

  • Calbindin likely plays a significant physiological role, possibly involving protein-protein interactions.
  • The evolutionary trajectory suggests independent duplications leading to calbindin and other calcium-binding proteins from a common ancestor.
  • A distinct calbindin-homologous gene exists in the brain, indicating further complexity in this protein family.