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Updated: Aug 7, 2025

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
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Using pressure-driven flow systems to evaluate laser speckle contrast imaging.

Colin T Sullender1, Adam Santorelli1, Lisa M Richards1

  • 1The University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas, United States.

Journal of Biomedical Optics
|March 14, 2023
PubMed
Summary
This summary is machine-generated.

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For reliable microfluidic flow phantom studies, pressure-regulated flow systems are superior to syringe pumps. Multi-exposure speckle imaging (MESI) provides more accurate flow measurements than laser speckle contrast imaging (LSCI).

Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Fluid Dynamics

Background:

  • Microfluidic flow phantom studies are essential for evaluating laser speckle contrast imaging (LSCI) instruments.
  • Accurate flow control is critical for reliable instrument performance characterization.
  • Syringe pumps are commonly used but may introduce flow uncertainties.

Purpose of the Study:

  • To compare the flow uncertainty of syringe pumps versus pressure-regulated flow systems.
  • To assess the performance of LSCI and multi-exposure speckle imaging (MESI) using a pressure-regulated system.
  • To determine the optimal flow control method for microfluidic LSCI studies.

Main Methods:

  • Evaluated syringe pump and pressure-regulated flow systems in a microfluidic device with an inline flow sensor.
Keywords:
dynamic light scatteringflow measurementflow systemlaser speckle contrast imagingmicrofluidicmulti-exposure speckle imaging

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  • Quantified flow uncertainty for each system under stepped flow profiles.
  • Characterized LSCI and MESI performance against the flow sensor using the pressure-regulated system.

    • Main Results:

    • Pressure-regulated systems demonstrated significantly more stable and reproducible flow than syringe pumps.
    • Syringe pumps exhibited 8x higher expanded uncertainty compared to pressure-regulated systems.
    • MESI outperformed LSCI across all tested flow speeds, with lower average errors than LSCI.

    Conclusions:

    • Pressure-regulated flow systems are recommended over syringe pumps for microfluidic flow measurement studies.
    • MESI provides superior accuracy for relative flow measurements compared to traditional LSCI.
    • Optimized flow control enhances the reliability of optical imaging instrument characterization.