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Updated: Aug 7, 2025
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
Tailoring the positive and negative solvatochromism for chalcone analogues to detect heterozygous protein
Bai Yulong1,2,3, Wan Wang1, Huang Yanan3,4
1CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. liuyu@dicp.ac.cn.
Abstract:
It is rare for one fluorophore scaffold to harbor both positive and negative solvatochromism. Herein, we tailor chalcone analogues to achieve both positive- and negative-polarity sensitivity of fluorescence intensity. We explore two chalcones of opposite solvatochromism to simultaneously detect the co-aggregation of wild-type and mutant superoxide dismutase that cause amyotrophic lateral sclerosis disease.
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