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MicroRNA-196a-5p targeting LRP1B modulates phenotype of thyroid carcinoma cells

Yaojie Hu1, Chunying Zhang1, Qing Chang1

  • 1Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.

Endokrynologia Polska
|March 14, 2023
PubMed
Abstract

Insights

MicroRNA-196a-5p is upregulated in thyroid cancer (TC) and promotes tumor progression by targeting LRP1B. Silencing this microRNA inhibits TC cell proliferation, invasion, and migration.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Thyroid cancer (TC) is a prevalent endocrine malignancy.
  • MicroRNAs (miRNAs) are increasingly recognized for their role in cancer progression.
  • The specific role of miRNAs in thyroid diseases requires further elucidation.

Purpose of the Study:

  • To investigate the regulatory mechanism of microRNA-196a-5p in thyroid cancer progression.
  • To determine if microRNA-196a-5p targets low-density lipoprotein receptor-associated protein 1B (LRP1B) in TC cells.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot to assess expression levels of microRNA-196a-5p and LRP1B.
  • Dual-luciferase reporter assay to confirm the targeting relationship.
  • Cell proliferation, invasion, and migration assays (CCK-8, scratch healing, Transwell) to evaluate functional effects.

Main Results:

  • MicroRNA-196a-5p expression was significantly upregulated in TC cells, while LRP1B expression was downregulated.
  • Upregulation of microRNA-196a-5p promoted TC cell proliferation, invasion, and migration.
  • Silencing microRNA-196a-5p reversed these effects, indicating its oncogenic role.
  • Dual-luciferase reporter assay confirmed that microRNA-196a-5p directly targets LRP1B.

Conclusions:

  • MicroRNA-196a-5p acts as a cancer-promoting microRNA in thyroid cancer.
  • The microRNA-196a-5p/LRP1B axis is a key regulatory pathway in TC progression.
  • MicroRNA-196a-5p represents a potential therapeutic target for thyroid cancer.
Keywords:
LRP1BinvasionmicroRNA-196a-5pmigrationproliferationthyroid carcinoma

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