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Related Experiment Videos

Oncogene expression in differentiating F9 mouse embryonal carcinoma cells.

T J Lockett1, M J Sleigh

  • 1CSIRO Division of Molecular Biology, New South Wales, Australia.

Experimental Cell Research
|December 1, 1987
PubMed
Summary

During F9 cell differentiation, oncogene RNA levels change, with c-myb decreasing early and c-src increasing later, potentially affecting growth rate. Retinoic acid influences c-abl RNA levels during this process.

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Area of Science:

  • Cellular and Molecular Biology
  • Developmental Biology
  • Cancer Research

Background:

  • F9 mouse embryonal carcinoma cells are a model for studying differentiation.
  • Cell differentiation involves significant changes in gene expression, including oncogenes.
  • Previous studies on oncogene expression during F9 cell differentiation have yielded varied results.

Purpose of the Study:

  • To investigate the dynamic changes in transcript levels of several c-oncogenes during F9 cell differentiation.
  • To correlate these oncogene expression changes with alterations in cell growth rate and tumorigenicity.
  • To examine the role of retinoic acid in modulating oncogene expression during differentiation.

Main Methods:

  • Culturing F9 cells in monolayer and aggregate formats to induce differentiation into parietal and visceral endoderm-like cells, respectively.

Related Experiment Videos

  • Analyzing transcript levels of c-myb, c-myc, c-src, c-abl, and c-fos oncogenes using RNA analysis.
  • Manipulating retinoic acid presence in the culture medium to assess its effect on oncogene expression.

    • Main Results:

    • Differentiation led to decreased growth rate and loss of tumorigenicity.
    • Qualitatively similar changes in several c-oncogene transcript levels were observed in both monolayer and aggregate cultures.
    • An early decrease in c-myb RNA and a later increase in c-src RNA were noted, potentially linked to reduced cell growth.
    • Elevated c-abl RNA levels were dependent on retinoic acid during induction and maintenance, with partial decline upon its removal post-differentiation.
    • Increased c-fos RNA was observed late in monolayer differentiation, mirroring embryonic development.

    Conclusions:

    • F9 cell differentiation involves specific, dynamic alterations in oncogene expression patterns.
    • These oncogene changes, particularly c-myb and c-src, may play roles in regulating cell growth during differentiation.
    • Retinoic acid is crucial for regulating c-abl expression during F9 cell differentiation.