Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.3K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.3K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.7K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.7K
The Extracellular Matrix01:29

The Extracellular Matrix

9.2K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Spatiotemporal Analysis of Intracellular Kinase Activity in Metastatic Breast Cancer Cells Using a Microfluidic-Based Lateral Diffusion Assay.

Analytical chemistry·2026
Same author

Fluid Forces Control Structural Remodeling of Blind-Ended Lymphatic Microvessels.

Advanced healthcare materials·2026
Same author

Nanobubbles, not microbubbles, enable ultrasound visualization of the perivascular space: A validation and focused ultrasound modulation study.

Ultrasonics·2026
Same author

Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.

ACS sensors·2026
Same author

Spatial Regulation of Endocytosis and Adhesion Formation Governs Breast Cancer Cell Migration Under Confinement.

Bioengineering (Basel, Switzerland)·2025
Same author

Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Aug 7, 2025

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

410

Extracellular Matrix-Derived Biophysical Cues Mediate Interstitial Flow-Induced Sprouting Angiogenesis.

Chia-Wen Chang1, Hsiu-Chen Shih2, Marcos G Cortes-Medina3

  • 1Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, United States.

ACS Applied Materials & Interfaces
|March 14, 2023
PubMed
Summary

Hyaluronan (HA) in collagen gels enhances blood vessel sprouting driven by interstitial flow. HA increases matrix stiffness and pore size, crucial for flow-mediated angiogenesis.

Keywords:
hyaluronidasemicrofluidicsmicrovessel analoguepore sizestiffnessvascular function

More Related Videos

Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

4.7K
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
10:47

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Published on: November 6, 2019

30.6K

Related Experiment Videos

Last Updated: Aug 7, 2025

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

410
Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

4.7K
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
10:47

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Published on: November 6, 2019

30.6K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Angiogenesis, the formation of new blood vessels, is regulated by biochemical and mechanical signals within the tissue microenvironment.
  • Interstitial flow and extracellular matrix (ECM) physical properties, like stiffness, are known regulators of angiogenesis.
  • The combined effects of interstitial flow and ECM properties on angiogenesis initiation remain unclear.

Purpose of the Study:

  • To investigate the interplay between interstitial flow and ECM physical properties in regulating angiogenic sprouting.
  • To determine the role of hyaluronan (HA) in modulating these interactions.

Main Methods:

  • Utilized a 3D microfluidic tissue model to simulate angiogenic sprouting.
  • Applied defined interstitial flow over collagen-based matrices with and without hyaluronan (HA).
  • Assessed matrix properties (stiffness, pore size) and sprouting responses, including experiments with CD44 blockade and hyaluronidase (HAdase) treatment.

Main Results:

  • Addition of HA to collagen matrices significantly enhanced interstitial flow-induced angiogenic sprouting compared to collagen-only matrices.
  • HA increased the stiffness and pore size of the collagen matrices.
  • Blocking the HA receptor CD44 did not affect sprouting, while enzymatic depletion of HA reversed the enhancement.

Conclusions:

  • Hyaluronan (HA) enhances interstitial flow-mediated angiogenic sprouting.
  • This enhancement is primarily attributed to HA's ability to alter collagen ECM stiffness and pore size, rather than through CD44 receptor signaling.