Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biotechnology

Background:

• Homogeneous immunosensors merge biosensor and immunoassay benefits, offering speed, sensitivity, and accuracy.
• Recent rapid development has led to cost-effective, user-friendly immunosensor technologies.
• These sensors find diverse applications across various fields.

Purpose of the Study:

• To review current immunosensor development trends.
• To focus on fluorescent and bioluminescent immunosensor technologies.
• To highlight advantages, improvements, and limitations of these immunosensors.

Main Methods:

• Literature review of recent advancements in immunosensor technology.
• Analysis of fluorescent immunosensor principles and applications.
• Analysis of bioluminescent immunosensor principles and applications.

Main Results:

• Homogeneous immunosensors offer significant advantages in detection speed and sensitivity.
• Fluorescent and bioluminescent immunosensors represent key areas of innovation.
• Specific strategies have been identified to address existing technological limitations.

Conclusions:

• Homogeneous immunosensors are a promising alternative to traditional diagnostic methods.
• Further development in fluorescent and bioluminescent systems will enhance their capabilities.
• Overcoming current limitations will expand the application scope of these advanced sensors.