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Related Concept Videos

Proteomics01:33

Proteomics

7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: Aug 6, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Hyperplexing Approaches for up to 45-Plex Quantitative Proteomic Analysis.

Zhen Wu1, Weirong Xiang1, Lin Huang1

  • 1State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China.

Analytical Chemistry
|March 14, 2023
PubMed
Summary
This summary is machine-generated.

A new tribrid hyperplexing approach combines three isobaric labeling reagents, including the novel isobaric tag 16-plex (IBT16), to expand quantitative proteomic analysis capacity up to 45-plex for large-scale studies.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Quantitative Biology

Background:

  • Isobaric labeling is crucial for quantitative proteomics, enabling high multiplexing.
  • Increasing sample sizes in cohort studies necessitate advanced hyperplexing strategies.

Purpose of the Study:

  • To introduce and evaluate a novel tribrid hyperplexing approach combining three isobaric labeling reagents.
  • To assess the performance of a new isobaric tag 16-plex (IBT16) reagent alongside TMT11 and TMTpro (TMT18) reagents.

Main Methods:

  • Developed a tribrid hyperplexing strategy using IBT16, TMT11, and TMTpro reagents.
  • Determined labeling efficiency and optimized testing conditions.
  • Evaluated peptide identification and quantification performance using E. coli and HeLa peptide mixtures.

Main Results:

  • The three isobaric labeling reagents demonstrated similar performance in identification and quantification.
  • Combinatorial use of the reagents expanded multiplexing capacity to up to 45-plex.
  • Identified advantages of IBT16 in combinatorial applications.

Conclusions:

  • The tribrid hyperplexing approach significantly enhances multiplexing capacity for quantitative proteomics.
  • IBT16 offers advantages for combinatorial use, with specific combinations recommended for practical applications.
  • This method supports large-scale proteomic analyses in cohort studies.