Evidence for a novel voltage-activated channel in the outer mitochondrial membrane

Patch-clamp studies of the outer mitochondrial membrane indicate a voltage-dependent increase in conductance for potentials positive relative to the exterior of the mitochondrion. The time course of the conductance changes is consistent with an activation of channels. Voltage pulse experiments suggest that the activation phenomenon corresponds to assembly of the channels from subunits with disassembly occurring after recovery of the original conductance. Effects of temperature and concanavalin A on the voltage-induced conductances are also consistent with a channel assembly model.