ELISA array-based immunoassay for multiplex detection of osteoporosis-relevant biomarkers

Aim: This study aimed to develop an ELISA array-based immunoassay for the simultaneous detection of osteoporosis-relevant biomarkers. Methods: ELISA array was constructed and its performance was evaluated using commercial antigens. The validity was further confirmed using traditional ELISA. Results: The constructed ELISA array showed good repeatability. The linear ranges and limits of detection for the four markers mentioned above were 0.01-8 ng/ml (1 pg/ml), 5.0-1000 ng/ml (1 ng/ml), 0.5-400 ng/ml (0.1 ng/ml) and 1-100 ng/ml (1 ng/ml), respectively. The measured concentrations of serum samples by ELISA array showed high correlation with those from traditional ELISA. Conclusion: ELISA array-based immunoassay provided a feasible and productive method to multiplex measure markers for osteoporosis.