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Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Subviral Agents01:29

Subviral Agents

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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Retroviruses

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Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
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Retrovirus Life Cycles01:10

Retrovirus Life Cycles

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Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
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Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

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Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
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Viral Recombination00:57

Viral Recombination

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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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Related Experiment Video

Updated: Aug 6, 2025

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Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

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Pseudotyped Viruses for Coronaviruses.

Meiyu Wang1,2, Jianhui Nie2, Youchun Wang3,4

  • 1Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

Advances in Experimental Medicine and Biology
|March 15, 2023
PubMed
Summary
This summary is machine-generated.

Pseudotyped viruses are essential for studying human coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Developing automated detection platforms enhances research efficiency for vaccines and therapeutics.

Keywords:
MERS-CoVMonoclonal antibodiesNeutralization assayPseudotyped virusSARS-CoVSARS-CoV-2Vaccine

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Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
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Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

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Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
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Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

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Area of Science:

  • Virology
  • Immunology
  • Biotechnology

Background:

  • Seven human coronaviruses exist, including four mild (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1) and three lethal (SARS-CoV, MERS-CoV, SARS-CoV-2).
  • Pseudotyped viruses are crucial research tools due to their safety, ease of preparation, detection, and modifiability.

Purpose of the Study:

  • To highlight the utility of pseudotyped viruses in human coronavirus research.
  • To explore the applications of pseudotyped viruses in understanding viral mechanisms, evaluating vaccines and therapeutics, and predicting viral evolution.
  • To emphasize the need for high-throughput, automated detection platforms for SARS-CoV-2 pseudotyped viruses.

Main Methods:

  • The abstract discusses the use of pseudotyped viruses as a research tool.
  • It implies various experimental and analytical methods are used in conjunction with pseudotyped viruses for evaluation and prediction.
  • The development of high-throughput platforms is mentioned as a methodological advancement.

Main Results:

  • Pseudotyped viruses facilitate research on virus infection mechanisms.
  • They are effective in evaluating vaccines, antiviral drugs, and antibodies.
  • Pseudotyped viruses serve as a platform for predicting immunogenicity, antigenicity, and cross-species transmission, and for developing broad-spectrum vaccines.

Conclusions:

  • Pseudotyped viruses are versatile tools in coronavirus research, offering safety and adaptability.
  • Their application extends to predicting viral evolution and developing improved vaccines.
  • There is a growing demand for automated, high-throughput detection systems for SARS-CoV-2 pseudotyped viruses to enhance efficiency and reduce costs.