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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Related Experiment Video

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Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
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Pseudotyped Viruses for Lyssavirus.

Wenbo Wang1, Caifeng Long1, Lan Wang2

  • 1Division of Monoclonal Antibody Products, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

Advances in Experimental Medicine and Biology
|March 15, 2023
PubMed
Summary

This study details the creation of pseudotyped rabies viruses (RABV) to investigate the functions of the G protein. These engineered viruses aid in evaluating rabies treatments and vaccines.

Keywords:
GlycoproteinLyssavirusPseudovirusRABV

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Lyssaviruses, including rabies virus (RABV), are bullet-shaped RNA viruses causing fatal rabies in warm-blooded animals.
  • The G protein of RABV is crucial for viral entry and is a key target for antiviral strategies.

Purpose of the Study:

  • To describe the construction of RABV G protein-bearing pseudotyped viruses.
  • To highlight the applications of these pseudotyped viruses in studying viral functions and evaluating anti-rabies products.

Main Methods:

  • Construction of pseudotyped viruses displaying RABV G protein.
  • Utilizing pseudotyped viruses for studying G protein biological functions.
  • Employing pseudotyped viruses for evaluating vaccine-induced antisera, rabies immunoglobulins, neutralizing monoclonal antibodies, and antiviral inhibitors.

Main Results:

  • Successfully constructed RABV G protein-bearing pseudotyped viruses.
  • Demonstrated the utility of these pseudotyped viruses in assessing the efficacy of various anti-rabies interventions.
  • Provided a platform for studying lyssavirus G protein functions.

Conclusions:

  • Pseudotyped RABV systems are valuable tools for understanding lyssavirus pathogenesis and for the development and evaluation of rabies countermeasures.
  • This approach facilitates research on both RABV and other related lyssaviruses.