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Published on: June 4, 2020
DNA labelling in live cells via visible light-induced [2+2] photocycloaddition
Rita T Michenfelder1,2, Laura Delafresnaye2, Vinh X Truong2,3
1Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber Weg 6, Karlsruhe 76131, Germany. wagenknecht@kit.edu.
Abstract:
We introduce a visible light-driven (λmax = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells via the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.
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