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Protocol for metabolic synergy analysis of multi-organs in mice using iMS2Net
Jiyang Dong1, Qianwen Peng1, Lingli Deng2
1Department of Electronic Science, National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen 361005, China.
STAR Protocols
|March 15, 2023
Summary
This study introduces imaging mass spectrometry (iMS) for in situ biomolecule detection, revealing spatial distributions. This method aids in understanding cellular and organ metabolic interactions for disease research.
Area of Science:
- Biochemistry
- Cell Biology
- Systems Biology
Background:
- Cellular and organ communication networks are crucial in disease.
- Understanding biomolecule spatial distribution is key to disease mechanisms.
Purpose of the Study:
- To detail a protocol for in situ biomolecule detection using imaging mass spectrometry (iMS).
- To establish a multiscale network strategy sourced from iMS data.
- To explore metabolic responses in environmental-exposure-induced disease models.
Main Methods:
- In situ detection of biomolecules using imaging mass spectrometry (iMS).
- Establishment of an iMS-dataset-sourced multiscale network strategy.
- Analysis of metabolic responses in disease models.
Main Results:
- iMS provides detailed spatial distribution of biomolecules.
- The multiscale network strategy effectively utilizes iMS data for metabolic interaction analysis.
- Metabolic responses in disease models can be explored using this approach.
Conclusions:
- iMS is a powerful tool for mapping biomolecule spatial distribution.
- The developed strategy enables comprehensive analysis of inter-cellular and inter-organ metabolic networks.
- This approach facilitates the study of metabolic dysregulation in environmentally induced diseases.
