Dairy wastewater treatment using Monoraphidium sp. KMC4 and its potential as hydrothermal liquefaction feedstock

Monoraphidium sp. KMC4 was cultivated mixotrophically for simultaneous treatment of dairy wastewater and biomass production. The KMC4 was cultivated with varying chemical oxygen demand concentrations of simulated synthetic dairy wastewater. Monoraphidium sp. KMC4 outperformed in 50% strength with biomass concentration of 1.47 g L^-1. A significant change in biomass of 3.69 g L^-1 was achieved after maintaining the pH of algal culture. The nutrient consumption promoted microalgal growth in the form of biomass productivity (122 mg L^-1 day^-1), accumulation of carbohydrate (28.73±1.6 wt%), protein (48.50±1.3 wt%), and lipid (20.29±2.3 wt%). This strain showed efficacious performance in treating simulated synthetic dairy wastewater obtaining biomass for various applications. The algal biomass derived from wastewater reported a significant volatile matter content and higher heating value. The biomass demonstrates satisfactory thermal degradation behavior which reveals its feasibility as feedstock for thermochemical conversion to biocrude. The integration of biomass production in high-scale raceway pond along with biocrude production is a promising pathway toward the generation of green energy for replacing traditional fossil fuels..