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Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

345
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
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Fertilization01:38

Fertilization

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During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
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A novel microfluidics-based device achieves automatic cumulus cells removal and optimal embryo development in cattle.

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Updated: Aug 6, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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The human cumulus cell transcriptome provides poor predictive value for embryo transfer outcome.

Álvaro Martínez-Moro1, Leopoldo González-Brusi2, Ismael Lamas-Toranzo2

  • 1Animal Reproduction Department, INIA, CSIC, Madrid, Spain; IVF Spain Madrid, Madrid, Spain.

Reproductive Biomedicine Online
|March 15, 2023
PubMed
Summary
This summary is machine-generated.

The cumulus cell transcriptome shows little correlation with pregnancy success. However, specific gene expression patterns in cumulus cells may predict an embryo's ability to reach the blastocyst stage.

Keywords:
Cumulus cellsDevelopmental competenceImplantation predictorOocyteRNA-seqTranscriptome

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Area of Science:

  • Reproductive biology
  • Genomics
  • Embryology

Background:

  • Cumulus cells, surrounding the oocyte, are crucial for oocyte maturation and fertilization.
  • Understanding the relationship between cumulus cell gene expression and embryo developmental competence is key for improving IVF outcomes.

Purpose of the Study:

  • To investigate whether the transcriptome of cumulus cells can predict an embryo's developmental competence and pregnancy potential.

Main Methods:

  • Cumulus cells were collected from human oocytes.
  • Transcriptome analysis was performed using RNA sequencing.
  • Samples were grouped based on oocyte developmental potential: non-blastocyst (Bl-), blastocyst but no pregnancy (P-), and blastocyst with pregnancy (P+).

Main Results:

  • The cumulus cell transcriptome was largely independent of pregnancy potential.
  • Few genes (10-11) were differentially expressed between pregnancy groups (P+ vs. P-, P+ vs. Bl-).
  • A subset of genes (e.g., CHAC1, CENPE, CD93, PECAM1, HSPA1B, EPN3) showed differential expression related to blastocyst development or pregnancy potential.

Conclusions:

  • Cumulus cell gene expression is largely unrelated to achieving clinical pregnancy.
  • Specific gene expression profiles in cumulus cells may indicate an embryo's potential to develop to the blastocyst stage.