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In vivo Neuronal Calcium Imaging in C. elegans

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Published on: April 10, 2013

Fast and sensitive GCaMP calcium indicators for imaging neural populations.

Yan Zhang¹, Márton Rózsa^1,2, Yajie Liang^1,3,4

¹Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

|March 16, 2023

Summary

Researchers developed new jGCaMP8 sensors for faster and more sensitive neural activity tracking. These protein-based calcium sensors enable observing large neuron populations at speeds relevant to brain computation.

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Subcellular Imaging of Neuronal Calcium Handling In Vivo

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Protein-based calcium indicators are crucial for monitoring neural activity in vivo.
Existing sensors face limitations in speed and sensitivity, hindering the study of rapid neural processes.

Purpose of the Study:

To develop and optimize novel GCaMP-type calcium indicators with improved kinetics and sensitivity.
To create sensors capable of tracking neural activity at timescales relevant to neural computation.

Main Methods:

Large-scale screening of protein variants.
Structure-guided mutagenesis of GCaMP-type indicators.
Biochemical and functional characterization of developed sensors.

Main Results:

Developed jGCaMP8 sensors with ultra-fast kinetics (2 ms half-rise time).
Achieved the highest sensitivity reported for protein-based calcium sensors.
Demonstrated suitability for tracking large neuronal populations at relevant timescales.

Conclusions:

jGCaMP8 sensors represent a significant advancement in calcium imaging technology.
These sensors overcome previous limitations, enabling more precise studies of neural dynamics.
The enhanced performance of jGCaMP8 will facilitate breakthroughs in understanding neural computation.