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The long noncoding RNA LINC15957 regulates anthocyanin accumulation in radish
Huping Tan1,2, Xiaobo Luo3, Jinbiao Lu1,2
1College of Agriculture, Guizhou University, Guiyang, China.
Frontiers in Plant Science
|March 16, 2023
Summary
A novel long noncoding RNA, LINC15957, was identified in radish. Overexpression of LINC15957 enhances anthocyanin accumulation and related gene expression, while silencing reduces it, revealing its role in pigment biosynthesis.
Area of Science:
- Plant Molecular Biology
- Agricultural Science
- Biochemistry
Background:
- Radish (Raphanus sativus L.) is a vital Brassicaceae crop, with anthocyanin-rich varieties prized for color and nutrition.
- The molecular mechanisms governing anthocyanin biosynthesis in radish skin and flesh remain largely unelucidated.
Purpose of the Study:
- To identify and characterize the role of long noncoding RNAs (lncRNAs) in regulating anthocyanin accumulation in radish.
- To investigate the function of a specific lncRNA, LINC15957, in controlling anthocyanin biosynthesis pathways.
Main Methods:
- Transient gene overexpression and silencing techniques were employed in radish.
- Transcriptome sequencing (RNA-Seq) was performed on LINC15957-overexpressed and control plants.
- Quantitative real-time PCR (RT-qPCR) was used to validate gene expression levels.
Main Results:
- Overexpression of LINC15957 significantly increased anthocyanin accumulation and the expression of related biosynthesis genes in radish leaves.
- Silencing LINC15957 led to reduced anthocyanin content and gene expression in radish flesh.
- Transcriptome analysis identified 5,772 differentially expressed genes, including key transcription factors (e.g., MYB, bHLH, WD40) involved in flavonoid biosynthesis pathways.
Conclusions:
- The lncRNA LINC15957 plays a crucial role in regulating anthocyanin biosynthesis in radish.
- This study provides valuable genetic resources and insights into the molecular regulation of anthocyanin accumulation in radish.
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