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Long-term toxicity study of Green S in mice
P G Brantom1, D M Creasy, I F Gaunt
1British Industrial Biological Research Association, Carshalton, Surrey, England.
Summary
Green S, a food colorant, showed no adverse effects in mice over a 2-year study. The highest dose tested, 0.66% of the diet, was determined to be the no-untoward-effect level for mice.
Area of Science:
- Toxicology
- Food Science
- Animal Studies
Background:
- Green S is a synthetic food dye used in various food products.
- Assessing the long-term safety of food additives is crucial for public health.
Purpose of the Study:
- To evaluate the potential toxicity of Green S in mice over a 2-year period.
- To determine the no-untoward-effect level (NOEL) of Green S in a rodent model.
Main Methods:
- Mice were fed diets containing 0%, 0.033%, 0.33%, or 0.66% Green S for up to 2 years.
- Reproductive parameters, body weight, clinical condition, hematology, and histopathology were monitored.
- Organ weights were recorded at necropsy.
Main Results:
- No significant differences were observed in reproductive outcomes, survival rates, or body weight between control and Green S-treated groups.
- Hematological and histopathological examinations revealed no treatment-related adverse effects.
- The highest dose tested (0.66%) did not cause any observable adverse effects.
Conclusions:
- The no-untoward-effect level of Green S in mice is at least 0.66% of the diet.
- Green S is considered safe for consumption at the tested levels over a 2-year period.