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Updated: Aug 6, 2025
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Published on: June 28, 2019
Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral
Xiao-Yan Tang1, Da Wang1, Xin-Yue Zhang1
1Institute for Stem Cell and Neural Regeneration, School of Pharmacy; State Key Laboratory of Reproductive Medicine; Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine; Nanjing Medical University, Nanjing, China.
Abstract:
Cerebral organoids represent an optimal experimental system for studying human cortical development, evolution, physiology, function, and disease mechanisms. Here, we describe a simple protocol for the differentiation of human pluripotent stem cells (hPSCs) into cerebral organoids. We describe steps for hPSC maintenance, neural induction of embryoid bodies, and patterning of cerebral organoids. We also detail a process for the phenotypic assay of each neural-tube-like area in hPSC-derived cerebral organoids. For complete details on the use and execution of this protocol, please refer to Tang et al. (2021).1.
