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Structure and expression of the human creatine kinase B gene
E C Mariman1, C A Broers, C A Claesen
1Department of Human Genetics, University Nijmegen, Radboud Hospital, The Netherlands.
Genomics
|October 1, 1987
Summary
Researchers isolated human creatine kinase type B (CK-B) cDNA, confirming its tissue-specific expression and relation to cell proliferation. A single CK-B gene was identified, with its structure characterized.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Creatine kinase type B (CK-B) is an important enzyme in cellular energy metabolism.
- Understanding the gene structure and expression patterns of CK-B is crucial for various biological processes.
Purpose of the Study:
- To isolate and characterize human creatine kinase type B (CK-B) cDNA.
- To investigate the tissue-specific expression of CK-B RNA.
- To determine the structure and organization of the human CK-B gene.
Main Methods:
- Isolation of human CK-B cDNA from cDNA libraries using oligonucleotide probes.
- Nucleotide sequencing of cDNA to determine the amino acid sequence.
- RNA analysis in various human tissues and cultured cells to measure CK-B gene activity.
- Genomic DNA screening and gene sequencing to elucidate gene structure.
Main Results:
- Isolation of an almost full-length human CK-B cDNA encoding a 381 amino acid polypeptide.
- High homology observed between human CK-B sequences and those of other vertebrates.
- CK-B RNA expression is tissue-specific and correlates with cell proliferation.
- Identification of a single human CK-B gene, approximately 3.2 kb in size.
- The CK-B gene is partially overlapped by an HTF island with high G+C content at its 5' end.
Conclusions:
- Human CK-B cDNA has been successfully isolated and characterized.
- CK-B gene expression is regulated in a tissue-specific manner, linked to cell proliferation.
- The human genome contains a single CK-B gene with a compact structure.