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Hypocretin-1 suppresses malignant progression of glioblastoma cells through Notch1 signaling pathway

Renzheng Huan1, Jianhe Yue1, Jinhai Lan2

  • 1Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Brain Research Bulletin
|March 16, 2023
PubMed

Insights

Hypocretin-1, a neuropeptide, effectively inhibits glioblastoma growth, migration, and invasion while promoting apoptosis. This antitumor effect is mediated by suppressing the NOTCH signaling pathway.

Area of Science:

  • Neuroscience
  • Oncology
  • Molecular Biology

Background:

  • Hypocretin-1 is a neuropeptide with known antitumor properties against various cancers.
  • Its role and mechanism in malignant glioma development remain largely uninvestigated.

Purpose of the Study:

  • To investigate the effects of hypocretin-1 on glioblastoma proliferation, apoptosis, migration, and invasion.
  • To elucidate the underlying molecular mechanism, focusing on the NOTCH signaling pathway.

Main Methods:

  • Assessed hypocretin-1 receptor expression in glioma cell lines and tissues.
  • Evaluated hypocretin-1 treatment effects on glioblastoma cell behavior in vitro.
  • Measured tumor growth rate and weight in vivo.
  • Investigated the role of the NOTCH signaling pathway, including NICD overexpression.

Main Results:

  • Hypocretin-1 receptor is expressed in glioma cells and tissues.
  • Hypocretin-1 inhibited glioblastoma cell proliferation, migration, and invasion, and induced apoptosis.
  • Hypocretin-1 significantly reduced tumor growth rate and weight.
  • Hypocretin-1's antitumor effects were mediated by inhibiting the NOTCH signaling pathway, as NICD overexpression reversed these effects.

Conclusions:

  • Hypocretin-1 demonstrates significant antitumor activity against glioblastoma.
  • The neuropeptide inhibits glioblastoma progression by suppressing the NOTCH signaling pathway.
  • Hypocretin-1 holds potential as a therapeutic agent for malignant glioma.
Keywords:
GlioblastomaHypocretin-1Malignant progressionNotch signaling pathway

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