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Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
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Fibroblast Growth Factor 2 Is Produced By Renal Tubular Cells to Act as a Paracrine Factor in Maladaptive Kidney

Xiaoru Hu1, Zhengwei Ma2, Siyao Li1

  • 1Department of Nephrology, Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital at Central South University, Changsha, People's Republic of China; Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University and Charlie Norwood VA Medical Center, Augusta, Georgia.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|March 16, 2023
PubMed

Insights

Fibroblast growth factor 2 (FGF2) is produced by kidney tubule cells after injury. This factor promotes kidney fibrosis and inflammation, contributing to kidney disease progression.

Area of Science:

  • Nephrology
  • Cell Biology
  • Molecular Medicine

Background:

  • Kidney repair involves complex cellular interactions, including communication between injured tubules, fibroblasts, and immune cells.
  • The precise roles of cytokines produced by tubular cells in kidney repair remain largely unclear.

Purpose of the Study:

  • To investigate the role of fibroblast growth factor 2 (FGF2) in kidney repair following injury.
  • To determine if FGF2 is induced by kidney injury and if it contributes to maladaptive repair processes.

Main Methods:

  • Induction of kidney injury in mice using repeated low-dose cisplatin (RLDC).
  • In vitro studies using RLDC-treated renal proximal tubule cells.
  • Assessment of FGF2 expression, secretion, and its effects on fibroblast proliferation and fibrosis-related protein expression.
  • Evaluation of kidney function, fibrosis, and inflammation in FGF2-deficient mice post-RLDC treatment.

Main Results:

  • RLDC treatment induced FGF2 expression and secretion in renal tubular cells.
  • RLDC-treated cell medium promoted fibroblast proliferation, an effect dependent on FGF2.
  • FGF2 contributed to the induction of fibrosis-related proteins.
  • FGF2 deficiency partially protected against RLDC-induced kidney dysfunction, fibrosis, and inflammation.

Conclusions:

  • Renal tubular cells produce FGF2 in response to kidney injury.
  • FGF2 acts as a paracrine factor that exacerbates kidney fibrosis and inflammation.
  • Targeting FGF2 may offer a therapeutic strategy for preventing maladaptive kidney repair and disease progression.
Keywords:
cisplatinfibroblast growth factor 2maladaptive kidney repairrenal fibrosis

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