Analysis of differentially expressed genes related to cerebral ischaemia in young rats based on the Gene Expression
1Department of Neurology, The Third People's Hospital of Hefei (The Third Clinical College of Anhui Medical University), Hefei 230022, Anhui Province, China.
Insights
Cerebral infarction in young people is rising. This study identified the cyclic adenosine monophosphate (c-AMP) signalling pathway as a key genetic pathway potentially involved in young-onset stroke, offering new intervention targets.
Area of Science:
- Neuroscience
- Genetics
- Molecular Biology
Background:
- Increasing incidence of cerebral infarction in younger populations.
- Complex pathogenesis and mechanisms of young-onset stroke pose treatment challenges.
- Genetic analysis is crucial for understanding young-onset cerebral infarction.
Purpose of the Study:
- Compare gene expression in young versus aged rats post-middle cerebral artery occlusion.
- Analyze the impact of differentially expressed genes on key signaling pathways in young rats.
- Identify genetic pathways involved in cerebral ischemia development in young individuals.
Main Methods:
- Utilized the Gene Expression Omnibus (GEO) 2R tool for analyzing the GSE166162 dataset.
- Employed DAVID 6.8 software for filtering differentially expressed genes.
- Conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.
Main Results:
- Identified 35 differentially expressed genes, including Igf2, Col1a2, and Sfrp1.
- GO analysis revealed involvement in drug response, blood vessel development, and signaling pathways.
- KEGG analysis pinpointed the cyclic adenosine monophosphate (c-AMP) signaling pathway as significantly enriched.
Conclusions:
- The c-AMP signaling pathway is a potential key pathway for intervening in young-onset cerebral infarction.
- Findings suggest genetic targets for therapeutic strategies in young stroke patients.
Background:
The incidence rate of cerebral infarction in young people is increasing day by day, the age of onset tends to be younger, and its internal pathogenesis and mechanism are very complicated, which leads to greater difficulties in treatment. Therefore, it is essential to analyze the key pathway that affects the onset of cerebral infarction in young people from the perspective of genetics.
Aim:
To compare the differentially expressed genes in the brain tissue of young and aged rats with middle cerebral artery occlusion and to analyse their effect on the key signalling pathway involved in the development of cerebral ischaemia in young rats.
Methods:
The Gene Expression Omnibus 2R online analysis tool was used to analyse the differentially expressed genes in the GSE166162 dataset regarding the development of cerebral ischaemia in young and aged groups of rats. DAVID 6.8 software was further used to filter the differentially expressed genes. These genes were subjected to Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to determine the key gene pathway that affects the occurrence of cerebral ischaemia in young rats.
Results:
Thirty-five differentially expressed genes (such as Igf2, Col1a2, and Sfrp1) were obtained; 73 GO enrichment analysis pathways are mainly involved in biological processes such as drug response, amino acid stimulation response, blood vessel development, various signalling pathways, and enzyme regulation. They are involved in molecular functions such as drug binding, protein binding, dopamine binding, metal ion binding, and dopamine neurotransmitter receptor activity. KEGG pathway enrichment analysis showed a significantly enriched pathway: The cyclic adenosine monophosphate (c-AMP) signalling pathway.
Conclusion:
The c-AMP signalling pathway might be the key pathway in the intervention of cerebral infarction in young people.
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