Superior sagittal sinus flow as a proxy for tracking global cerebral blood flow dynamics during wakefulness and sleep

Sleep, a state of reduced consciousness, affects brain oxygen metabolism and lowers cerebral metabolic rate of oxygen (CMRO₂). Previously, we quantified CMRO₂ during sleep via Fick's Principle, with a single-band MRI sequence measuring both hemoglobin O₂ saturation (SvO₂) and superior sagittal sinus (SSS) blood flow, which was upscaled to obtain total cerebral blood flow (tCBF). The procedure involves a brief initial calibration scan to determine the upscaling factor (f_c), assumed state-invariant. Here, we used a dual-band sequence to simultaneously provide SvO₂ in SSS and tCBF in the neck every 16 seconds, allowing quantification of f_c dynamically. Ten healthy subjects were scanned by MRI with simultaneous EEG for 80 minutes, yielding 300 temporal image frames per subject. Four volunteers achieved slow-wave sleep (SWS), as evidenced by increased δ-wave activity (per American Academy of Sleep Medicine criteria). SWS was maintained for 13.5 ± 7.0 minutes, with CMRO₂ 28.6 ± 5.5% lower than pre-sleep wakefulness. Importantly, there was negligible bias between tCBF obtained by upscaling SSS-blood flow, and tCBF measured directly in the inflowing arteries of the neck (intra-class correlation 0.95 ± 0.04, averaged across all subjects), showing that the single-band approach is a valid substitute for quantifying tCBF, simplifying image data collection and analysis without sacrificing accuracy.