Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization

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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

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Hikari Seo1, Shiro Murata1,2, Osamu Ichii3,4

  • 1Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.

The Journal of Veterinary Medical Science
|March 17, 2023
PubMed

Keywords:
RNAscopecathepsin D-1cystatinin situ hybridizationpoultry red mites

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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