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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Updated: Aug 6, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Analyzing CRISPR screens in non-conventional microbes.

Varun Trivedi1, Adithya Ramesh1, Ian Wheeldon1,2,3

  • 1Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.

Journal of Industrial Microbiology & Biotechnology
|March 17, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR screens advance functional genomics by identifying genes linked to desired traits. This study details methods for microbial CRISPR screens to improve bioprocessing and discover new targets.

Keywords:
CRISPR screeningFunctional genomicsGenetic screen analysisGenotype–phenotype relationshipsNon-conventional yeasts

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Area of Science:

  • Functional genomics
  • CRISPR technology
  • Microbial biotechnology

Background:

  • CRISPR screens are powerful tools in functional genomics, widely used in mammalian cells for disease research and drug development.
  • Their application in non-model microbes for bioprocessing improvements is limited due to a lack of specialized bioinformatic tools.
  • This gap hinders the discovery of novel metabolic engineering targets in industrially relevant microorganisms.

Conclusions:

  • Pooled CRISPR screens offer significant potential for advancing functional genomics in non-model yeasts.
  • This approach can uncover novel metabolic engineering targets for enhanced industrial bioproduction.
  • Further development of bioinformatic tools will facilitate broader application in microbial biotechnology.