The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect

The root barrier to radial O₂ loss (ROL) is a key root trait preventing O₂ loss from roots to anoxic soils, thereby enabling root growth into anoxic, flooded soils. We hypothesized that the ROL barrier can also prevent intrusion of hydrogen sulphide (H₂ S), a potent phytotoxin in flooded soils. Using H₂ S- and O₂ -sensitive microsensors, we measured the apparent permeance to H₂ S of rice roots, tested whether restricted H₂ S intrusion reduced its adverse effects on root respiration, and whether H₂ S could induce the formation of a ROL barrier. The ROL barrier reduced apparent permeance to H₂ S by almost 99%, greatly restricting H₂ S intrusion. The ROL barrier acted as a shield towards H₂ S; O₂ consumption in roots with a ROL barrier remained unaffected at high H₂ S concentration (500 μM), compared to a 67% decline in roots without a barrier. Importantly, low H₂ S concentrations induced the formation of a ROL barrier. In conclusion, the ROL barrier plays a key role in protecting against H₂ S intrusion, and H₂ S can act as an environmental signalling molecule for the induction of the barrier. This study demonstrates the multiple functions of the suberized/lignified outer part of the rice root beyond that of restricting ROL.